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Culture and enrichment method, medium and maintenance of cancer stem cells

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Partner keyword: 
Cytology, Cancerology, Oncology
In-vitro diagnostics


A Spanish hospital research group has developed a cell culture medium suitable for the isolation and/or enrichment of cancerous stem cells (CSCs). This technology does not require previous manipulation of cells and it is serum free. The research group is seeking to establish a licensing agreement or an agreement of a public-private partnership in order to further develop the technology (technical cooperation agreement).



Cancer is, after heart disease, the second most common cause of death in the developed countries nowadays. Cancer stem cells (CSCs) are cancer cells (found within tumors) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample.

A Spanish research group from the public health service, whose field of activity is, among others, medical oncology, supported by two universities, has developed a cell culture medium for isolating and/or enriching cancer stem cells, produced by culturing of mesenchymal stem cells in a conventional serum-free medium.

The patented technology consists in sowing, in the previous conditioned medium, a sample containing CSCs, and producing the cell population, with the additional characteristic that, before culturing the CSCs in the conditional medium, the culture is treated with diluted trypsin, and the cells, which are sensitive to trypsin and have a higher percentage of CSCs, are isolated.

The technology is serum free, which promotes the in vitro proliferation and conservation of pluripotency potential which allows to maintain an undifferentiated state of cancer stem cells subpopulation and at the same time does not allow the survival of differentiated cells.

The group is looking for the following collaboration:
-License agreement, in the framework of authorizing the interested parties to use the technology.
-Technical cooperation agreement, to collaborate with other research teams, companies or foundations.

Advantages & innovations

Cooperation plus value: 
- Serum-free conditioned medium that does not require prior manipulation of cells. - Efficient enrichment method of cancer stem cells (CSCs), as well as obtaining a population basically pure of CSCs.

Stage of development

Cooperation stage dev stage: 
Available for demonstration

Partner sought

Cooperation area: 
The research group aims to formalize a technology transfer process with entities interested in the implementation of the solution, through a license agreement. If there are some entities, such as R&D centres, technology centres or specialized companies, interested in collaborating and developing further aspects of the solution, a technical cooperation agreement could be envisaged.

Type and size

Cooperation task: 
SME 11-50,University,R&D Institution,SME <10,>500 MNE,251-500,SME 51-250,>500