The German Centre is part of an independent and free research institution that has the legal status of a non-profit limited liability company. Its portfolio comprises, e.g. process and product development, manufacturing, prototyping and scientific technical services including experimental testing and analyses. The enormous expertise is bundled at the Centre.
One of the main working fields is the development of technology and hardware for cold applications in several fields of tissue engineering, cryobiology, cryomedicine and pharmaceutical freezing as well as testing and analytics. The client offers large experiences in processing and manufacturing 3-D biomaterials with customized structures, pore sizes and cell specific additives on basis of biopolymers, e.g. hyaluronic acid and collagen.
Due to rheological measurement in a wide temperature range, a complete characterization of the dispersions, e.g. pastes, gels and polymers, is possible. The determination of the yield point or non-Newtonian behavior from measured flow curves, the temperature depending viscosity or thixotropic behavior could be determined. The stability of the dispersion over time or the linear viscoelastic range could be determined with oscillatory measurement.
A second part is the determination of dispersed (solid) particles in solution by using different measurement ranges and flow through cuvettes. The physical measurable sizes of particles could be adapted to their general form, e.g. diameter or perimeter of a circle with an equivalent area for approximately spherical particles, fibre length for fibrous material or Feret diameter for irregular shapes. To describe the particles, the shape can be determined, e.g. aspect ratio, convexity or sphericity. For elongated particles like fibres, length and straightness can be determined. For every imaged particle its parameters can be described separate.
With this features it is possible to detect single particles which don’t match the requirements, e.g. in a production batch, or to prove an evidence for agglomeration.
Furthermore, the Centre is developing histological methods for biological and technical materials. The process steps can be customized in close agreement with the client to get the best results. The Centre offers different methods of dewatering, penetration and embedding, e.g. paraffin or liquid plastic embedding, and modern cutting techniques down to 5 µm. With a wide range of staining techniques, depending on material or cell population, and optical as well as fluorescence microscopic imaging nearly every material can be evaluated.
The broad expertise of the German client ranges from HE-stained oral mucosa over Masson-Goldner stained bone tissue to fluorescence dying methods and to damage analysis of synthetic polymers and preparation of difficult materials.
The examination of product and transport safety of packaging materials under cryogenic conditions is another working field. With innovative methods, the quantity of carbon dioxide penetrating the packaging material or the seal can be identified as well as the tightness in contact with liquid nitrogen under storage conditions.
Cooperation in terms of a services agreements:
The client is looking for partners to solve their:
- Technical challenges and measurements
- Problems in the characterization
- Problems in production of dispersions
- Problems with tightness/ transport safety of packaging materials, which are used for storage under cryogenic environment.
- Problems with successful cryopreservation of tissues or of replacement models for animal testing.
Cooperation in terms of subcontracting:
The Centre offers also its expertise as project partner or subcontractor in public funded research or industrial research.