The organization is a Spanish biotechnological private non-profit research institute, founded in 1993, whose main activities are related to molecular biology and microbial biotechnology. The institution keeps close links with industrial research and innovation. The team of experienced researchers covers several techniques and counts with a long experience in scientific research in European funding projects.
The reprogram microorganism technologies for biological sensors offered are:
- gene synthesis
- promoters and terminators selection and modification
- microbial genome modification (e.g.: electroporation, conjugation, CRISPR, …)
- gene regulation (e.g.: transcriptional regulators, smRNA)
Genomics, metagenomics, transcriptomics and metatranscriptomics
- identification and phylogenetic classification of microbial isolates based on PCR amplification and sequencing of SSU rRNA regions,
- shotgun metagenomics analysis,
- whole genome sequencing of pure isolates using Illumina and PacBio platforms,
- transcriptomics analysis using Agilent custom microarrays (own designs) and RNA-seq technology,
- RNA-seq metatranscriptomics analysis
- Sample preparation, purification, cleaning from different (micro)organisms or fluids
- Visible (Coommassie, Silver) and fluorescent staining (DIGE, Pro-Q Diamond, SYPRO Ruby …)
- 2D-Western detection
- Protein identification by mass spectrometry (MALDI-TOF/TOF).
Cultivation and isolation of microorganisms
- Microbial (fungi, yeast, bacteria) isolation from natural sources
- Taxonomical location
-Microbial manufacturing capabilities development [growth conditions optimization, metabolites production (antibiotics, carotenoids, …)]
- Raw materials degradation
- Scale-up (5, 20, 300 l)
- Metabolites production analysis
The institute, looking for private and public partners (companies, research institutions and universities), would like to collaborate on joint research and development projects, under research cooperation agreements. In the short term this call has been identified: “Reprogrammed microorganisms for biological sensors” (BIOTEC-06-2020).