Enterprise Europe Network

Reprogram microorganism technology for biological sensors

Country of origin:
Country: 
SPAIN
Opportunity:
External Id: 
TOES20191205003
Published
05/12/2019
Last update
18/12/2019
Expiration date
18/12/2020

Keywords

Partner keyword: 
Genetic Engineering
Synthetic Biology
Microbiology
Bioinformatics
Bioprocesses
Cellular and Molecular Biology
Genetic Engineering
Microbiology
Bioinformatics
Gene Expression, Proteome Research
Research and experimental development on biotechnology
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Summary

Summary: 
A Spanish biotechnological research institute offers expertise and equipment in synthetic biology on industrially relevant microorganisms (Fungi and Actinobacteria), omics analysis, isolation and characterization of microorganisms, protein purification and characterization, and process development of microbial high-value products. A research cooperation agreement with companies, research centres or universities is sought. Collaboration in European funding programs will be welcome.

Description

Description: 

The organization is a Spanish biotechnological private non-profit research institute, founded in 1993, whose main activities are related to molecular biology and microbial biotechnology. The institution keeps close links with industrial research and innovation. The team of experienced researchers covers several techniques and counts with a long experience in scientific research in European funding projects.

The reprogram microorganism technologies for biological sensors offered are:

Synthetic Biology
- gene synthesis
- promoters and terminators selection and modification
- microbial genome modification (e.g.: electroporation, conjugation, CRISPR, …)
- gene regulation (e.g.: transcriptional regulators, smRNA)

Genomics, metagenomics, transcriptomics and metatranscriptomics
- identification and phylogenetic classification of microbial isolates based on PCR amplification and sequencing of SSU rRNA regions,
- shotgun metagenomics analysis,
- whole genome sequencing of pure isolates using Illumina and PacBio platforms,
- transcriptomics analysis using Agilent custom microarrays (own designs) and RNA-seq technology,
- RNA-seq metatranscriptomics analysis
Proteomics
- Sample preparation, purification, cleaning from different (micro)organisms or fluids
- Visible (Coommassie, Silver) and fluorescent staining (DIGE, Pro-Q Diamond, SYPRO Ruby …)
- 2D-Western detection
- Protein identification by mass spectrometry (MALDI-TOF/TOF).

Cultivation and isolation of microorganisms
- Microbial (fungi, yeast, bacteria) isolation from natural sources
- Taxonomical location
-Microbial manufacturing capabilities development [growth conditions optimization, metabolites production (antibiotics, carotenoids, …)]
- Raw materials degradation
- Scale-up (5, 20, 300 l)
- Metabolites production analysis

The institute, looking for private and public partners (companies, research institutions and universities), would like to collaborate on joint research and development projects, under research cooperation agreements. In the short term this call has been identified: “Reprogrammed microorganisms for biological sensors” (BIOTEC-06-2020).

Advantages & innovations

Cooperation plus value: 
The centre has long tradition in European Union funded projects (FP6, FP7, H2020, ERA-NETs …) and international collaborations. The centre accumulate more than 25 year of knowledge in genetic engineering and synthetic biology focused on nutritional regulation (e.g.: phosphate, stringent response …) and secondary metabolites production and regulation (e.g: feedback, transcriptional regulators, smRNAs …). Besides, the omics knowledge allows the monitoring the whole metabolic processes (genome sequencing, transcriptomics, proteomics, metabolomics). The analytic and fermentation facilities guarantee the microbial culture process from the initial microbial isolation and characterization (e.g.: own microbial culture collection from different environments) to the final scale up process (e.g.: 5, 20, 300 litres fermenters …). The described skills allow the centre integration in different steps of biosensors design and validation process.

Stage of development

Cooperation stage dev stage: 
Under development/lab tested

Partner sought

Cooperation area: 
The institute is interested in contacting with industries, academia or research organizations to join a project as a partner in order to submit a proposal to H2020 subprogramme: biotechnology on reprogrammed microorganisms for biological sensors (BIOTEC-06-2020). The partnership considered is research cooperation agreement.

Type and size

Cooperation task: 
SME 11-50,University,R&D Institution,SME <10,>500 MNE,251-500,SME 51-250,>500