At present, both nucleic acid detection and quantification play an essential role in medicine, veterinary science, microbiology, pharmacology or toxicology, being decisive in the diagnosis, control, monitoring and treatment of numerous pathologies. Current biochemical and culture-based techniques have low sensitivity, reliability and accuracy and take too long to provide conclusive results. That is the reason why today molecular techniques are widely used, even though most of them require additional verification by sequencing analysis for definitive confirmation of presumptive positive results (presence of a polymerase chain reaction - PCR- product that matches the expected size after an electrophoresis assay). In any case, these techniques entail a high expenditure on equipment, reagents, consumables, service outsourcing, sample storage and transportation and the time needed to obtain and interpret analytical results, all of which greatly increases the cost of testing.
A Spanish SME dedicated to developing cutting-edge products in the field of molecular diagnostics and with broad expertise in biotechnology and molecular biology has developed a proprietary state-of-the-art device, which allows specific capture and detection of genetic sequences.
The technology of the device is based on scientifically-proven molecular principles and on the use of super fluorescent and metastable molecules for robust detection of specific DNA sequences and genetic diagnosis. This allows for fast, reliable and selective identification of nucleic acids without performing neither electrophoresis nor sequencing assays, which reduces both analysis duration and its economic cost. Moreover, it is possible to accurately quantifying the DNA concentration of a sample in a few minutes, verifying the purity and integrity of said sample, and maintaining high sensitivity and affordable costs in comparison to current alternatives. In summary, the technology can be used to perform several nucleic-acid-based tests:
• Analytical and diagnostic PCR reading
• Analysis of genetic markers associated to clinically-relevant microorganisms and specific phenotypic traits
• Genotyping tests (detection of synthetic molecular barcodes, genetic profiling and characterization…)
• Quantitative and expression analysis
The analysis device is a portable multimodular gadget with different functional units integrated in an innovative design that allows for the optimization of the entire analytical process. Each of these modules focuses on specific activities of the experimental procedure and can be programmed to perform different functions both together and separately through a general control interface.
The SME is looking for a distribution services agreement.
As a result of the intended collaboration, the SME expects to associate with a partner capable of gradually achieving a broad implementation of the technology in an international market. Accomplishing this will allow for a progressive development of new analytical applications based on said platform, thus diversifying the product portfolio and maximizing market value.