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In vitro bio-compatibility studies and physio-chemical characterization of bio-materials are offered under services agreement.

Summary

Profile Type
  • Technology offer
POD Reference
TOES20250205006
Term of Validity
5 February 2025 - 5 February 2026
Company's Country
  • Spain
Type of partnership
  • Research and development cooperation agreement
Targeted Countries
  • All countries
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General information

Short Summary
A Spanish research team, specialized in the field of cell therapy and tissue engineering, offers an ‘in vitro’ testing service of specific bio-molecules or new bio-materials for a first screening of possible biological effects. In addition a physio-chemical characterization of medical devices (implants, stents), bio-materials and pharmaceuticals is also performed. Research groups, universities and public or private companies interested in services agreement are being sought.
Full Description
A team belonging to a Spanish Research Institute on Health Sciences and specialized in the R&D of new therapies and technologies in the field of cell therapy, tissue engineering and regenerative medicine, with a proven track record in biocompatibility studies and in physicochemical characterization of biomaterials offers the following services:

1. Biocompatibility tests:
• Cytotoxicity (LDH Activity or cell viability tests).
• Assays of cell metabolism and proliferation.
• Cell morphology by Scanning Electron/Confocal microscopy.
• Cell adhesion profile
• Gene expression profiling by real-time RT-PCR.
• Release of specific markers by immunoassays.
• Functional assays: ALP activity and mineralization (osteoblasts), resorption pit assay (osteoclasts), wound healing assay (gingival fibroblasts), collagen production…
• Hemocompatibility tests: Hemolysis, Platelet adhesion.

Tests can be conducted using the following (a) in vitro reconstructed tissue models, validated by the Scientific Committee on Consumer Safety (SCCS) as an alternative for corrosion and tissue irritation tests for cosmetic products. These models are sourced from certified providers. (b) in vitro cell cultures: human Bone Marrow Mesenchymal Stem Cells, human gingival fibroblasts, human peripheral blood mononuclear cells, mouse preosteoclast and pre-osteoblast cell lines, human vascular endothelial cells. Other cell lines can be set up upon demand. Other assays can also be set up upon demand.

All studies are performed in compliance with the guidelines established by the regulatory agencies. Protocols are designed to meet the regulatory requirements of current standards (ISO 10993: Biological Evaluation of Medical Devices).

2. Physicochemical characterization:
Characterization studies with several types of biomaterials can be performed: Implant surfaces (functionalized, coated, modified…), Hydrogels, Scaffolds and porous structures, Micro and nanoparticles (solids or suspensions).
Both bulk and surface characterizations of metallic and polymeric biomaterials are also offered:
• Surface chemistry and composition of biomaterials.
• Topography and morphology.
• Release profile of bioactive molecules from biomaterials.
• Stability in body fluids.

The following techniques are usually applied: UV-Vis spectroscopy, fluorescence spectroscopy, FTIR spectroscopy, fluorescence microscopy, FTIR microscopy (solids and surfaces), HPLC, ICP-AES, SEM, SEM-EDX (morphology at the micro-scale, elemental composition mapping), AFM (nanotopography). Although other techniques are also available through partners: contact angle, profilometry, micro-CT, XPS, ToF-SIMS, particle size distribution, Z-potential.

Universities and public or private companies in the field of cell therapy and tissue engineering interested in a service agreement are being sought.
Advantages and Innovations
In vitro tests are done with the aim of reducing the use of animals and the costs related to in vivo analysis. The research team has a proven track record in biocompatibility studies and in physicochemical characterization of biomaterials therefore they can offer high quality results with low turnaround time.
Stage of Development
  • Already on the market
Sustainable Development Goals
  • Goal 3: Good Health and Well-being
IPR status
  • IPR granted

Partner Sought

Expected Role of a Partner
Pharmaceutical companies, medical device producers or research institutions interested in biocompatibility tests or physicochemical characterization of biomaterials are being sought. The type of collaboration offered is a services agreement.
Type and Size of Partner
  • SME 50 - 249
  • SME <=10
  • SME 11-49
  • Big company
  • University
  • R&D Institution
Type of partnership
  • Research and development cooperation agreement

Dissemination

Technology keywords
  • 06002002 - Cellular and Molecular Biology
  • 06002007 - In vitro Testing, Trials
Market keywords
  • 04006 - Cellular and Molecular Biology
  • 04005 - Biochemistry / Biophysics
Targeted countries
  • All countries